Published PCR primers targeting the ammonia monooxygenase gene (amoA) were applied to samples from activated sludge systems operated with low dissolved oxygen (DO) to quantify total and clade-level Nitrospira that perform complete ammonium oxidation (comammox); however. we found these existing primers resulted in significant artifact-associated non-target amplification. This not only ... https://www.aitsglobal.com/product-category/%d7%a2%d7%99%d7%a6%d7%95%d7%91-%d7%94%d7%91%d7%99%d7%aa/