Single-molecule and bulk biophysical approaches to study protein-DNA interactions on surface-immobilized nucleic acid templates typically rely on modifying the ends of linear DNA molecules to enable surface-DNA attachments. Unless both strands are constrained. this results in topologically free DNA molecules and the inability to observe supercoiling-dependent biological processes or r... https://www.spidertattooz.com/OPI-Infinite-Shine-Combo-Base-Top-Indefinitely-Baby-ISL55-p26708/