ABSTRACT Due to the general symptoms presented by the Chikungunya virus (CHIKV)-infected patients. a laboratory test is needed to differentiate CHIKV from other viral infections. The reverse transcription-quantitative real-time PCR (RT-qPCR) is a rapid and sensitive diagnostic tool. and several assays have been developed for detecting and quantifying CHIKV. https://www.opheliasmuse.com/