Site-specific N-glycosylation characterization requires intact N-glycopeptide analysis based on suitable tandem mass spectrometry (MS/MS) method. Electron-transfer/higher-energy collisional dissociation (EThcD). stepped collision energy/higher-energy collisional dissociation (sceHCD). higher-energy collisional dissociation-product-dependent electron-transfer dissociation (HCD-pd-ETD). https://www.diegojavierfares.com/